METHYL ETHYL KETONE (M.E.K.) (Urine) HPLC
Methyl Ethyl Ketone (Urine) For HPLC
INTENDED USE
KR144 Reagent kit for the HPLC determination Methyl Ethyl Ketone
KIT SPECIFICATION
Contents for 100 analyses
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Cat no
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Quantity
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Reagents
|
|
KR1441
|
1000 mlx1
|
Mobile Phase
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|
KR1443
|
0.5 mlx5
|
Urine Calibrator
|
|
KR1444
|
5.0mlx4
|
Derivatization solution
|
|
KR1445
|
2.5mlx1
|
Hydrolysis Reagent
|
ACCESSORIES
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KR1440
|
1 adet
|
HPLC column for the analysis of Methyl Ethyl Ketone
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|
KR1449
|
100 adet
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Autosampler vial with teflon caps
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CONTROLS
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KR1447
|
0.5 ml x5
|
Urine Control, Level I
|
|
KR1448
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0.5 ml x5
|
Urine Control, Level II
|
INTRODUCTION
Methyl Ethyl Ketone has no colour liquid with a sweet/sharp, acetone-like odour and fragrant. It is an extremely combustible liquid and vapour which is heavier than air and may spread long distant combustion and flashback are possible. M.E.K. has highly volatility. It is irritating to eyes and aspiration tract of central nervous system depressant. High vapour concentrations may induce headache, dizziness, nasusea, drowsiness, confusion and incoordination. M.E.K. also causes eye irritation and it is a possible reproductive hazard-may caused fetotoxicity based on animal data.
M.E.K. is found in some fruits and vegetables and happens naturally in blood, urine and exhaled air as a minor product of normal metabolism.
The M.E.K. TLV for an exposition of 8 h is 200 ppm.
EQUIPMENT AND INSTRUMENTAL PARAMETERS
The analysis of Methyl Ethyl Ketone requires a HPLC system including isocratic pump, autosampler and UV detector.
The use of a solvent degasser prevents air bubbles and ensure a stable baseline.
Sampler: Injection volume 50 μl, analytical run time 20 min..
HPLC pump: Flow rate 1.2 ml/min is recommended
Column temperature: Ambient (~25 °C)
UV detector: 334 nm
COLLECTION AND STORAGE OF PATIENT SAMPLES
Before and soon after work shift, collect 10 ml of urine in a suitable test tube.
The sample is stable up to 4 weeks at - 20 °C.
SAMPLE PREPARATION PROCEDURE
200 μl urine sample is added to vial with teflon cap
20 μl hydrolysis reagent is added.
Incubation is carried out at 100°C for 60 min.
Vortex the mix for 10s and then Centrifuge at 4 000 rpm for 5 min
200 μl hydrolysis reagent is added.
10 μl internal standard is added.
Incubation is carried out at 70°C for 30 min.
Samples prepared as above can be stored at ambient temperature for about 24 hours, at +2 to +8 °C up to 36 h, frozen below -18 °C up to 3 months.
